NASC

NASC ID: N850098

Name: WiscDsLox263F07

ABRC stock number: CS850098

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox263F07.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G11540.1 and AT4G11550.1 (chr 4 pos 6995776 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797158.

Wiscseq_DsLox263F07.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G55710.1 and AT1G55720.1 (chr 1 pos 20826208 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797156.

Wiscseq_DsLox263F07.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G07750.1-UTR3 and AT2G07760.1 (at chr 2 pos 3583180 (C/3583180-3583257) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 85-100 bps onto the T-DNA. Pools P 1, R 0, C 5 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452688.

Wiscseq_DsLox263F07.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G18692.1 (chr 4 pos 10284411 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797159.

Wiscseq_DsLox263F07.5 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G10040.1-UTR3 and AT4G10050.1-UTR3 (chr 4 pos 6280533 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797160.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox263F07

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G07750 AT2G07760